Antifungal effects of Zataria multiflora Boisss (Shirazi Thyme) extract against Aspergillus fumigatus Afs35 genes: crza and rpda

Aspergillus fumigatus causes a fatal infection particularly among immunocompromised individuals. Nowadays herbal and medicinal essential oils play vital role as an alternative safe medication. Zataria multiflora Boiss have antifungal activities mainly on the expression of some gene required for fungal survival. The main objective study was evaluation effects Z. extract against A. AfS35 strain. strain obtained from department biology, Baghdad University. Various concentrations (10-300mg/mL) were prepared minimum inhibitory concentration (MIC) fungicidal (MFC) determined using micro-broth dilution method. crzA rpdA genes evaluated quantitative real-time PCR (RT-qPCR) technique. ≥100mg/mL inhibited growth which non-toxic to normal cell line (MIC=100mg/mL MFC=150mg/mL). combination 50mg/mL plus each amphotericin B (10mg/ml) nystatin (10mg/mL) significantly decreased MIC level (p<0.001). 10mg/mL 2.4 (p<0.001) 3.6 (p<0.0001) fold, respectively. same non- by 0.6 (p=0.224) 1.1 (p=0.033) concluded that exerted effect inhibiting wall biosynthesis (down-regulation crzA), sustainability virulence (rpdA). therapy with multifora is promising eradicate infections.